Le risque infectieux viral chez le polytransfusé : séroprévalence de sept agents viraux dans le centre tunisien Viral infection risk in polytransfused adults: seroprevalence of seven viruses in central Tunisia

The aim of this study is to evaluate the prevalence of seven transfusion-transmitted viruses in polytransfused adults and children comparatively with a group of healthy control subjects. We studied 107 polytransfused patients (59 adults and 48 children) and 160 control subjects (100 blood donors and 60 children). Immunoenzymatic tests were used for detection of HBs antigen (HBs Ag), antibodies against hepatitis C Virus (anti-HCV), and human immunodeficiency virus (anti-HIV), and IgG antibodies against human cytomegalovirus (IgG anti-CMV), human parvovirus B19 (IgG anti-PB19), and hepatitis E virus (IgG anti-HEV). An immunofluorescent assay was performed for the detection of human herpesvirus 8 antibodies (anti-HHV8). Prevalence of HBs Ag, anti-HCV, anti-HIV, IgG anti-CMV, IgG antiPB19, IgG anti-HEV, and anti-HHV8 in polytransfused group was 8.4, 4.7, 0, 86.9, 60.7, 28.9, and 47.6%, respectively, and 1.8, 0.6, 0, 86.2, 53.1, 10, and 12.5%, respectively, in the control group. The difference in prevalence between the two groups was statistically significant for HBs Ag (P = 0.01), anti-HCV (P= 0.03), IgG anti-HEV (P < 10), N. Hannachi (*) · M. Marzouk · M. Tfifha · J. Boukadida Laboratoire de microbiologie–immunologie, UR02SP13, CHU Farhat-Hached, avenue Ibn-Jazzar, 4000 Sousse, Tunisie e-mail : [email protected] L. Boughammoura · S. Soussi Service de pédiatrie, CHU Farhat-Hached, avenue Ibn-Jazzar, 4000 Sousse, Tunisie A. Khlif Service d’hématologie, CHU Farhat-Hached, avenue Ibn-Jazzar, 4000 Sousse, Tunisie H. Skouri Laboratoire d’hématologie et banque du sang, 03/UR/08–18, CHU de Sahloul, 4000 Sousse, Tunisie Bull. Soc. Pathol. Exot. DOI 10.1007/s13149-010-0103-7